A modification of the "Sickledex" test for hemoglobin S.

Rapid solubility test for detection of hemoglobin S.

Several screening methods for detecting sickling hemoglobins are presently used in the clinical laboratory (1-6). The present procedure depends on the insolubility of reduced hemoglobin S in a 2.24 mol/liter phosphate buffer. The commercially available “Sickledex” (Ortho Diagnostics, Raritan, N.J. 08869) is also thought to be based on this principle (6). Our method was compared with the standar...

Spot test for detection of sickling hemoglobin.

We describe a simple, noninstrumental procedure for detecting hemoglobin S in samples of blood on filter paper, with use of small volumes of reagents. The method depends on the differential solubilities of reduced sickling and nonsickling hemoglobin in phosphate solutions (1, 2) or Sickledex reagent’ and the apparent solubilizing effect of urea on reduced hemoglobin S (3). The color of paired p...

Modified Sickledex tube test: a specific test for S hemoglobin.

A report form for the serial display of laboratory results in small hospitals.

Because Sickledex, like the sickling test, only detects sickle haemoglobin it is imperative that electrophoresis is performed on all positive results. At the same time the rare false positive results due to a dysproteinaemia will be detected. This test is valuable, particularly in the laboratory, where occasional sickling tests are performed and positive control cells are difficult to obtain. L...

An assessment of Sickledex as an alternative to the sickling test.